Participation of glutamatergic and nitrergic systems in the striatal dopamine release induced by isatin, a MAO inhibitor

Isatin is a biofactor with different biochemical and pharmacological properties whose effects attract much attention because it an endogenous inhibitor of the monoamine oxidase in brain. When exogenously administrated, isatin increases dopamine levels intact denervated striatum rats, effect that could indicate its potential as therapeutic agent Parkinson disease. However, neurochemical mechanisms by which are poorly understood. In present study, we evaluate role glutamatergic nitrergic systems isatin-induced release from rat striatum. Our findings show intrastriatal administration 10 mM significantly vivo (1,104.7% ± 97.1%), amino acids glutamate (428.7% 127%) taurine (221% 22%) measured brain microdialysis. The pretreatment MK-801 (500 µM) or AP5 (650 (glutamatergic NMDA receptors antagonists) reduces on 52% 70.5%, respectively. nitric oxide synthase inhibitors, L-NAME (100 7-NI also decreases 77% 42%, These results isatin, addition to increasing release, levels, possibly activates production, can promote further increase release.